Association of Oxidative Damage Measured by 8-hydroxyguanosine Formation with Altered Risks to Hepatocellular Carcinoma in Malaysian Study Population

@#Introduction: Oxidative damage is an important factor contributing to ageing and many degenerative diseases. It can be detected by the DNA base damage, which is formation of 8-oxo-7,8-dihydro-2’deoxyguanosine (8-oxodG). The 8-oxodG is an important indicator of oxidative stress and has been competently specified as a recognized initiator of the carcinogenic process and premutagenic injury in mammalian cells. Aims: In this preliminary study, we investigated the possible association of oxidative DNA damage in hepatocellular carcinoma (HCC) patients in comparison with Malaysian healthy controls taking into account the different races and genders in both groups. Method: DNA of peripheral white blood cells was isolated from 91 HCC patients and 304 controls. The level of oxidative DNA damage was determined by ELISA procedure. Results: Quantitative measurement of 8-oxodG was higher in HCC patients at mean value of 3.30 ± 2.32 ng/ml. In controls, the average value is 1.57 ± 1.92 ng/ml. Comparison between gender showed that there was a significant difference observed in the level of 8-oxodG between male and female in controls, where p = 0.003. The level of 8-oxodG was higher in male than in female controls. There was a significant difference in the average value of 8-oxodG level between the controls and HCC patients where p<0.001. However, no significant difference in the level of 8-oxodG value was observed when compared between Malays and the non-Malays. Conclusion: HCC patients showed greater oxidative damage to DNA as compared to controls and this suggests oxidative DNA damage may contribute to the pathogenesis of HCC.

Cardiac Related Mortality Trends in Forensic Department of Hospital Serdang, Selangor, Malaysia

@#Introduction: Cardiovascular disease (CVD) is the principal cause of admission and death in the Malaysian government hospitals. Method: The pattern of cardiac related mortality (CRM) cases in Hospital Serdang, Selangor was reviewed to determine the specific trends in ethnicity, age, gender and type of CRM. Data was drawn from the death registry records in Forensic Department of Hospital Serdang from January 2006 to December 2008. This preliminary project was carried out to assess the number of CRM cases and their characteristics to feed the decision to sensibly embark on another project related to atherosclerosis. Results: Out of 573 CRM cases, 84.5% were Malaysians out of which the majority were Malays (45.9%), followed by Chinese (30.2%), and Indians (22.1%). The majority were men (78.4%) with a mean age of death at 51.83 ± 14.10 (95% CI 50.52-53.14) years as compared to women (21.6%) with a mean age of death at 57.42 ± 16.92 (95% CI 54.41-60.43) years. Declining mortality trend was observed only in men. The 46-60 years old age group had the highest percentage of mortality and continually increase by year. Ischaemic heart disease (IHD) was the most common CRM type (69.1% in 2006, 66.2% in 2007, and 71.9% in 2008). Conclusion: We observed inconsistent trends of CRM in Hospital Serdang in terms of ethnicity, age, gender and type of CRM cases presented. Nevertheless, these findings do not necessarily represent the overall trends of CRM in Malaysia.

Biochemical Effects of Statins on Lipid Parameters among Newly Diagnosed Dyslipidaemia Subjects in Primary Care

@#Introduction: Statins have several pleiotropic effects including its primary effect of lipid lowering that is important to prevent cardiovascular disease (CVD). Subjects often have heterogeneous responses to statin. This study aims to determine the biochemical effects of statins on lipid parameters among newly diagnosed dyslipidaemia subjects. Methods: This was a prospective observational study involving 118 newly diagnosed adults with dyslipidaemia from three government health clinics in Selangor, Malaysia. Biochemical analyses including fasting lipid profile [triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C)] and apolipoproteins (apoA1, apoB) were taken at baseline and follow-up after a month on statin. Results: Majority of subjects (61.9%) were prescribed with lovastatin, with the rest on simvastatin. At baseline, the median values for all lipid profile parameters (TC, LDL-C, HDL-C) and non-conventional lipid parameters (LDL-C:HDL-C ratio, non-HDL-C, TC:HDL-C ratio, apoB:apoA1 ratio) were deranged except for TG and apoA1. On follow up, all parameters showed median values within the reference range except for HDL-C, non-HDL-C and TC:HDL-C ratio. There was significant difference in the effect of statins on lipid parameters including predictors of cardiovascular risk, simvastatin having better effects. Conclusions: Different statinshave varying effects on lipid parameters. Simvastatin showed significantly better effects compared to lovastatin. Non-HDL value should be included in the standard lipid profile report given its ease of use and implementation as it’s both a marker of coronary artery disease (CAD) risk stratification as well as an established determinant of goal attainment during therapy.

Clinical presentation and microorganisms sensitivity profile for diabetic foot ulcers: a pilot study

SUMMARY Introduction: Patients suffering from diabetes mellitus (DM) frequently present with infected diabetic foot ulcers (DFU). This study was done to record the anatomical site and the grade of ulcers according to Wagner’s classification and to culture the microorganisms from the ulcers and determine their antibiotic sensitivity. Materials and methods: Prospective study was conducted on 77 diabetic patients who were admitted with DFU from June until December 2011. Patients with end stage renal failure, those who had previous vascular surgery on the involved limb, or hyperbaric oxygen or maggot therapy for the ulcers, or had unrelated skin diseases around the involved foot were excluded from the study. Specimens for culture were obtained by a sterile swab stick or tissue sample was taken from the wound with sterile surgical instruments. Results: Wagner’s grade III and IV ulcers were most common. Majority of the ulcers involved toes (48%). Gram negative microorganisms were predominantly isolated (71.1%). Gram positive microorganisms were less frequently cultured (27.7%). Fungus was cultured from one sample (1.2%). Gram negative microorganisms were sensitive to aminoglycosides, cephalosporins or β-lactamase inhibitors. More than 40% were resistant to ampicillin. Gram positive microorganisms were sensitive to cloxacillin. MRSA were sensitive to vancomycin. Conclusion: Empirical use of antibiotics should be curtailed to prevent development of drug resistant strains of microorganisms and MRSA. We suggest use of antiseptic solutions to clean the ulcers until antibiotic sensitivity report is available. Results of our altered treatment regimen we plan to publish in a later study.

Clinical Presentation of Congenital Adrenal Hyperplasia in Selected Multiethnic Paediatric Population

A clinical descriptive study was done to determine the sociodemographic, laboratory and clinical characteristics of patients with congenital adrenal hyperplasia (CAH) referred to Hospital Putrajaya, a tertiary endocrine centre in Malaysia. Electronic laboratory data of 51 CAH patients were obtained. The demographics and clinical details of the study population were acquired from a questionnaire completed by parents of participants. There were 25 males (49%) and 26 females (51%), of which, 58.8% were Malays. Median age of participants was 4 years whilst median age at diagnosis of CAH was two years. Parental consanguinity was documented in three patients (5.9%). Patients originated from Johor (19.6%), Selangor (19.6%), Negeri Sembilan (17.6%) and Kedah (13.7%). Majority of patients were diagnosed after one week of life (80.4%) although more females were diagnosed under the age of one week compared to males (p=0.041). Most females presented with ambiguous genitalia (42.3%) [p=0.001] whereas 72% of males presented with salt wasting (p=0.003). No significant associations between race and all other variables, though interestingly three Malay patients presented with ambiguous genitalia and hypertension. Equal gender distribution noted as expected in an autosomal recessive condition, although not in keeping with other Asian countries. Early diagnosis in females attributed to obvious genital ambiguity at birth. Varied clinical presentation, although in minority, necessitates genetic studies for prompt diagnosis and treatment. Considering that majority of patients presented with salt wasting and the age at diagnosis was delayed, the introduction of a neonatal screening programme is essential in Malaysia.